Diagnostic performance of panfungal PCR on tissue specimens for the diagnosis of invasive fungal diseases: a systematic review and meta-analysis of the Fungal PCR Initiative (FPCRI).
J Clin MicrobiolAug 21, 2026 (epub)
Paraskevas Filippidis, Céline El Khoury, Mario Cruciani et al.
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In 30 seconds
This systematic review and meta-analysis evaluated the diagnostic performance of panfungal PCR on tissue specimens for invasive fungal diseases. Analyzing 28 studies, it found that in formalin-fixed paraffin-embedded (FFPE) samples, the sensitivity was 75.4% and specificity was 93.5%. In non-fixed samples, sensitivity was higher at 86.5%, demonstrating superior performance compared to culture methods.
Key findings
- In FFPE samples, panfungal PCR sensitivity was 75.4% and specificity was 93.5%.
- Sensitivity in non-fixed samples was 86.5%, with insufficient data for specificity.
- Panfungal PCR sensitivity was significantly higher than culture methods (88.2% vs 52.2%, P = 0.001).
- Interstudy variability was noted, indicating a need for standardized protocols.
Why it matters
The findings support the use of panfungal PCR as a reliable diagnostic tool for invasive fungal diseases, particularly in conjunction with other diagnostic methods. Improved sensitivity in non-fixed samples may enhance early detection and treatment.